Macroenzyme detection by polyethylene glycol precipitation
نویسندگان
چکیده
منابع مشابه
Precipitation of DNA by polyethylene glycol and ethanol.
The use of polyethylene glycol (PEG) for precipitation of DNA has been described in the literature (1-6) . We present here conditions for precipitation of DNA by PEG that give improved recoveries of DNA so that precipitation by PEG is comparable to that by ethanol, in terms of recovery, over a wide range of DNA concentrations. The problem of precipitation of lowmolecular-weight DNA fragments by...
متن کاملMacroprolactin detection by precipitation with protein A-sepharose: a rapid screening method compared with polyethylene glycol precipitation.
within 21 days. The likelihood ratio for a negative result [(1 sensitivity)/specificity] was 0.59 for birth within 7 days, 0.68 for birth within 14 days, and 0.53 for birth within 21 days. The data in Table 1 are similar to those obtained in previous studies using the ELISA-based assay. Joffe et al. (1 ) reported a NPV of 99.6% and a PPV of 9.1% for 243 patients who delivered within 7 days with...
متن کاملPolyethylene glycol precipitation as a screening method for macroprolactinemia.
To the Editor: Vieira et al. (1) used the Wallac Delfia immunofluorometric assay to demonstrate that macroprolactin is a common cause of apparent hyperprolactinemia, and this confirms our experience (2) and that of others (3). However, their data validating the polyethylene glycol (PEG) precipitation as a screening method for detecting macroprolactinemia are substantially different than ours (2...
متن کاملUltrafiltration – an alternative method to polyethylene glycol precipitation for macroprolactin detection
INTRODUCTION The aim of the study was to evaluate two methods of macroprolactin (MaPRL) detection - precipitation with polyethylene glycol (PEG) and ultrafiltration and to compare these techniques with "gold standard" - gel filtration chromatography (GFC). MATERIAL AND METHODS The study was conducted on 245 patients - 45 with organic and 200 with functional hyperprolactinaemia. In all the sub...
متن کاملCharacteristics of precipitation-formed polyethylene glycol microgels are controlled by molecular weight of reactants.
This work describes the formation of poly(ethylene glycol) (PEG) microgels via a photopolymerized precipitation reaction. Precipitation reactions offer several advantages over traditional microsphere fabrication techniques. Contrary to emulsion, suspension, and dispersion techniques, microgels formed by precipitation are of uniform shape and size, i.e. low polydispersity index, without the use ...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 2003
ISSN: 0004-5632,1758-1001
DOI: 10.1258/000456303322326425